Plastid targeting of E. coli β-glucuronidase and ADP-glucose pyrophosphorylase in maize (Zea mays L.) cells

@article{Russell1993PlastidTO,
  title={Plastid targeting of E. coli β-glucuronidase and ADP-glucose pyrophosphorylase in maize (Zea mays L.) cells},
  author={Douglas A. Russell and David L. DeBoer and David M. Stark and Jack Preiss and Michael E. Fromm},
  journal={Plant Cell Reports},
  year={1993},
  volume={13},
  pages={24-27}
}
Dicot and monocot chloroplast targeting peptides (CTPs) were evaluated for their effect on targeting, processing, and expression of two reporter proteins in maize cells. When tested transiently in maize leaf protoplasts, the maize ribulose bisphosphate carboxylase small subunit CTP required the inclusion of the amino terminus of mature small subunit protein to target β-glucuronidase (GUS) to the plastid. To remove this amino terminal extension from GUS after import and processing, a repeat of… CONTINUE READING