Plastid Protease Activity and Prolamellar Body Transformation during Greening.

  title={Plastid Protease Activity and Prolamellar Body Transformation during Greening.},
  author={Ruediger Hampp and L F De Filippis},
  journal={Plant physiology},
  volume={65 4},
Two proteases active in and specific to oat etioplasts and up to 24-hour etiochloroplasts, only very slightly contaminated by other cellular compartments are described. The enzyme showed pH optima of 4.2 (acid) and 6.8 (neutral), temperature optima of 50 C and the highest level of enzyme activity was with prolamellar bodies (PLBs) as substrate. Both enzymes showed evidence of a sulfhydryl reagent requirement, particularly for the neutral enzyme. Levels of both proteases increased up to 4 hours… CONTINUE READING

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