Plasticity of Cultured Mesenchymal Stem Cells: Switch from Nestin‐Positive to Excitable Neuron‐Like Phenotype

  title={Plasticity of Cultured Mesenchymal Stem Cells: Switch from Nestin‐Positive to Excitable Neuron‐Like Phenotype},
  author={Sabine Wislet-Gendebien and Gr{\'e}gory Hans and Pierre Leprince and Jean-Michel Rigo and Gustave Moonen and Bernard Rogister},
  journal={STEM CELLS},
Bone marrow mesenchymal stem cells (MSCs) can differentiate into several types of mesenchymal cells, including osteocytes, chondrocytes, and adipocytes, but, under appropriate experimental conditions, can also differentiate into nonmesenchymal cells—for instance, neural cells. These observations have raised interest in the possible use of MSCs in cell therapy strategies for various neurological disorders. In the study reported here, we addressed the question of in vitro differentiation of MSCs… 

Human placenta-derived mesenchymal stem cells acquire neural phenotype under the appropriate niche conditions.

The ability of human placenta-derived MSCs to differentiate into a neural phenotype in coculture assays with astrocytes obtained from neonatal rats demonstrates that placental stem cells may be able to differentiation into neural cell types when in direct contact with a neural environment.

Role of mesenchymal stem cells in neurogenesis and nervous system repair

Functional Neuronal Differentiation of Bone Marrow‐Derived Mesenchymal Stem Cells

It is demonstrated for the first time that clonal mMSCs and their progeny are competent to differentiate along the neuronal pathway, demonstrating that these bone marrow‐derived stem cells share characteristics of widely multipotent stem cells unrestricted to mesenchymal differentiation pathways.

Regulation of nestin expression by thrombin and cell density in cultures of bone mesenchymal stem cells and radial glial cells

It is demonstrated that thrombin stimulates the growth of radial glial cells and mesenchymal stem cells (MSCs) in a dose-dependent manner and that nestin expression by MSCs and RG is regulated in opposite manner by Thrombin in vitro.

Neuronal differentiation potential of human adipose-derived mesenchymal stem cells.

A-MSC may be a ready source of adult MSC with neuronal differentiation potential, an useful tool to treat neurodegenerative diseases.

Brain mesenchymal stem cells: The other stem cells of the brain?

The recent finding that brain cancer stem cells can transdifferentiate into pericytes is another facet of the plasticity of these cells, suggesting that the perversion of the stem cell potential ofpericytes might play an even unsuspected role in cancer formation and tumor progression.

Methodology, biology and clinical applications of mesenchymal stem cells.

C cultured MSCs were found to secrete various bioactive molecules that display anti-apoptotic, immunomodulatory, angiogenic, anti-scarring, and chemoattracted properties, providing a basis for their use as tools to create local regenerative environments in vivo.



Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells

It is demonstrated that cultured adult rat stromal cells in culture can express nestin, an intermediate filament protein predominantly expressed by neural stem cells, and it is suggested that nestin expression by these cells might be a prerequisite for the acquisition of the capacity to progress towards the neural lineage.

Plasticity of marrow-derived stem cells.

Only after a better understanding of the mechanisms involved and of the cells required for this differentiation will the authors be able to fully harness adult stem cell plasticity for clinical purposes.

Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains.

MSCs are capable of producing differentiated progeny of a different dermal origin after implantation into neonatal mouse brains and are potentially useful as vectors for treating a variety of central nervous system disorders.

Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats--similarities to astrocyte grafts.

The results suggest that MSCs may be useful vehicles for autotransplantation in both cell and gene therapy for a variety of diseases of the central nervous system.

Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells.

This analysis sets the stage for directing differentiation of human ES cells in culture and indicates that multiple human cell types may be enriched in vitro by specific factors.

Differentiation of Human Embryonic Stem Cells into Embryoid Bodies Comprising the Three Embryonic Germ Layers

The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.

Adult stem cell plasticity: fact or artifact?

  • M. Raff
  • Biology
    Annual review of cell and developmental biology
  • 2003
There has been unprecedented recent interest in stem cells, mainly because of the hope they offer for cell therapy, and examples of plasticity where differentiated cells or their precursors can be reprogrammed by extracellular cues to alter their character in ways that could have important implications for cell Therapy and other forms of regenerative treatment.

Cell transplantation of peripheral-myelin-forming cells to repair the injured spinal cord.

Recent findings that examine the remyelination potential of transplantation of peripheral-myelin-forming cells and progenitor cells derived from brain and bone marrow are discussed.

The transcription factor Sox10 is a key regulator of peripheral glial development.

It is shown that the transcription factor Sox10 is a key regulator in differentiation of peripheral glial cells in mice that carry a spontaneous or a targeted mutation of Sox10, and that it controls expression of ErbB3 in neural crest cells.