Plastic-Adherent DNA Aptamer-Magnetic Bead and Quantum Dot Sandwich Assay for Campylobacter Detection

@article{Bruno2008PlasticAdherentDA,
  title={Plastic-Adherent DNA Aptamer-Magnetic Bead and Quantum Dot Sandwich Assay for Campylobacter Detection},
  author={John Gordon Bruno and Taylor Phillips and Mar{\'i}a Paz Carrillo and Randy Crowell},
  journal={Journal of Fluorescence},
  year={2008},
  volume={19},
  pages={427-435}
}
DNA aptamers were developed against MgCl2-extracted surface proteins from Campylobacter jejuni. The two highest affinity aptamers were selected for use in a magnetic bead (MB) and red quantum dot (QD)-based sandwich assay scheme. The assay was evaluated using both heat-killed and live C. jejuni and exhibits detection limits as low as an average of 2.5 colony forming unit (cfu) equivalents in buffer and 10–250 cfu in various food matrices. The assay exhibits low cross-reactivity with bacterial… CONTINUE READING
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