Plasmonic Scattering Imaging of Single Proteins and Binding Kinetics

@article{Zhang2020PlasmonicSI,
  title={Plasmonic Scattering Imaging of Single Proteins and Binding Kinetics},
  author={Pengfei Zhang and Guangzhong Ma and Wei Dong and Zijian Wan and Shaopeng Wang and Nongjian Tao},
  journal={Nature methods},
  year={2020},
  volume={17},
  pages={1010 - 1017}
}
Measuring the binding kinetics of single proteins represents one of the most important and challenging tasks in protein analysis. Here we show that this is possible using a surface plasmon resonance (SPR) scattering technique. SPR is a popular label-free detection technology because of its extraordinary sensitivity, but it has never been used for imaging single proteins. We overcome this limitation by imaging scattering of surface plasmonic waves by proteins. This allows us to image single… Expand

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