Plasmon-enhanced stimulated Raman scattering microscopy with single-molecule detection sensitivity

  title={Plasmon-enhanced stimulated Raman scattering microscopy with single-molecule detection sensitivity},
  author={Cheng Zong and Ranjith Premasiri and Haonan Lin and Yimin Huang and Chi Zhang and Chen Yang and Bin Ren and Lawrence D. Ziegler and Ji‐Xin Cheng},
  journal={Nature Communications},
Stimulated Raman scattering (SRS) microscopy allows for high-speed label-free chemical imaging of biomedical systems. The imaging sensitivity of SRS microscopy is limited to ~10 mM for endogenous biomolecules. Electronic pre-resonant SRS allows detection of sub-micromolar chromophores. However, label-free SRS detection of single biomolecules having extremely small Raman cross-sections (~10−30 cm2 sr−1) remains unreachable. Here, we demonstrate plasmon-enhanced stimulated Raman scattering (PESRS… 

Pulse-picking coherent anti-Stokes Raman scattering microscopy for highly sensitive chemical imaging

Coherent Raman scattering has been developed into powerful technologies for the chemical imaging of biological samples. However, limited sensitivity remains a critical bottleneck for coherent Raman

Coherent Raman scattering microscopy for chemical imaging of biological systems

Coherent Raman scattering (CRS) processes, including both the coherent anti-Stokes Raman scattering and stimulated Raman scattering, have been utilized in state-of-the-art microscopy platforms for

Quantitative Drug Dynamics Visualized by Alkyne-Tagged Plasmonic-Enhanced Raman Microscopy.

The results illustrate that alkyne-tag SERS microscopy has a potential to be an alternative bioorthogonal imaging technique to investigate temporal dynamics of small-molecule uptake of live cells for pharmaceutical research.

Mammalian cell and tissue imaging using Raman and coherent Raman microscopy

An overview of RS modalities and techniques for mammalian cell and tissue imaging, with a focus on the advances and applications of CARS and SRS microscopy, for a better understanding of the metabolism and dynamics of lipids, protein, glucose, and nucleic acids in mammalian cells and tissues.

Nonlinearity-modulated single molecule trapping and Raman scattering analysis.

A new degree of freedom is demonstrated for effectively modulating the optical trapping and Raman detection in single molecule level in a tip-enhanced Raman scattering (TERS) system.

Quantum-enhanced nonlinear microscopy.

By showing that the photodamage limit can be overcome, this work will enable order-of-magnitude improvements in the signal-to-noise ratio and the imaging speed.

Time-Domain Observation of Surface-Enhanced Coherent Raman Scattering with 105-106 Enhancement.

Time-domain Raman measurements of trans-1,2-bis(4-pyridyl)ethylene (BPE) adsorbed on gold nanoparticle assemblies (GNAs) are reported, which were carried out with impulsive stimulated Raman spectroscopy using sub-8 fs pulses and provide a technical basis for femtosecond time-resolved SE-ISRS experiments to track ultrafast dynamics of the adsorbates.

Enhancement of Molecular Coherent Anti-Stokes Raman Scattering with Silicon Nanoantennas.

Surface-enhanced coherent anti-Stokes Raman scattering (SE-CARS) takes advantage of surface plasmon resonances supported on metallic nanostructures to amplify the coherent Raman response of target



Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy

Three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS) is reported, which offers background-free and readily interpretable chemical contrast in biomedical imaging and shows a variety of biomedical applications.

Coherent anti-Stokes Raman scattering with single-molecule sensitivity using a plasmonic Fano resonance.

It is shown that coherent anti-Stokes Raman spectroscopy (CARS) can be used to obtain single-molecule detection sensitivity, by exploiting the unique light harvesting properties of plasmonic Fano resonances.

Microsecond Scale Vibrational Spectroscopic Imaging by Multiplex Stimulated Raman Scattering Microscopy.

Microsecond scale vibrational spectroscopic imaging by lock-in free parallel detection of spectrally dispersed stimulated Raman scattering signal is reported, which opens new opportunities for compositional analysis of cellular compartment in a microscope setting and high-throughput spectral profiling of single cells in a flow cytometer setting.

Electronic Preresonance Stimulated Raman Scattering Microscopy

  • Lu WeiWei Min
  • Chemistry
    The journal of physical chemistry letters
  • 2018
The physical origin and uniqueness of the electronic preresonance region is discussed, followed by quantitative analysis of the enhancement factors involved in EPR-SRS microscopy, and an outlook for future development as well as the broad applications in biophotonics is provided.

Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.

A sensitive technique for vibrational imaging of tissues by combining coherent anti-Stokes Raman scattering (CARS) with video-rate microscopy is developed and CARS imaging and spectroscopy of lipid-rich tissue structures in the skin of a live mouse is demonstrated, with unprecedented contrast at subcellular resolution.

Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering

In vivo SRS imaging in living animals and humans is enabled by substantially enhancing the collection of the backscattered signal and increasing the imaging speed by three orders of magnitude to video rate, which allows label-free in vivo imaging of water, lipid, and protein in skin and mapping of penetration pathways of topically applied drugs in mice and humans.

Hyperspectral imaging with stimulated Raman scattering by chirped femtosecond lasers.

It is demonstrated that quantitative concentration determination of cholesterol in the presence of interfering chemical species can be achieved with sensitivity down to 4 mM, and it is shown that mammalian cell SRS hyperspectral imaging reveals the spatially inhomogeneous distribution of saturated lipids, unsaturatedlipids, cholesterol, and protein.

Chemical mapping of a single molecule by plasmon-enhanced Raman scattering

Raman spectral imaging with spatial resolution below one nanometre is demonstrated, resolving the inner structure and surface configuration of a single molecule by spectrally matching the resonance of the nanocavity plasmon to the molecular vibronic transitions, particularly the downward transition responsible for the emission of Raman photons.

Coherent Raman Scattering Microscopy

Coherent Raman Scattering Microscopy is an optical imaging method rooted in Raman Scattering. It involves Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS). CARS and

Spectrometer-free vibrational imaging by retrieving stimulated Raman signal from highly scattered photons

In vivo vibrational imaging reveals vitamin E distribution on mouse skin in vivo and captures human breast cancer tissues in situ and development of deep-tissue Raman spectroscopy toward molecular level diagnosis is demonstrated.