Plasmid transformation by electroporation of Leuconostoc paramesenteroides and its use in molecular cloning.

@article{David1989PlasmidTB,
  title={Plasmid transformation by electroporation of Leuconostoc paramesenteroides and its use in molecular cloning.},
  author={S T David and Guus Simons and Willem M de Vos},
  journal={Applied and environmental microbiology},
  year={1989},
  volume={55 6},
  pages={1483-9}
}
In this report, we demonstrate the utility of electroporation as an efficient method for genetic transformation of Leuconostoc paramesenteroides. We optimized several factors which determine the transformation frequency, resulting in transformation efficiencies of up to 4 x 10(3) transformants per micrograms of pNZ12 DNA, which contains the promiscuous Lactococcus lactis pSH71 replicon. Slightly lower efficiencies were obtained with a deletion derivative of the broad-host-range plasmid pAM beta… CONTINUE READING
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