Plasmid-mediated uptake and metabolism of sucrose by Escherichia coli K-12.

  title={Plasmid-mediated uptake and metabolism of sucrose by Escherichia coli K-12.},
  author={Katharina Schmid and M Schupfner and Ruediger Schmitt},
  journal={Journal of bacteriology},
  volume={151 1},
The conjugative plasmid pUR400 determines tetracycline resistance and enables cells of Escherichia coli K-12 to utilize sucrose as the sole carbon source. Three types of mutants affecting sucrose metabolism were derived from pUR400. One type lacked a specific transport system (srcA); another lacked sucrose-6-phosphate hydrolase (scrB); and the third, a regulatory mutant, expressed both of these functions constitutively (scrR). In a strain harboring pUR400, both transport and sucrose-6-phosphate… CONTINUE READING


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