Plasmid-based short-hairpin RNA interference in the chicken embryo.

@article{Chesnutt2004PlasmidbasedSR,
  title={Plasmid-based short-hairpin RNA interference in the chicken embryo.},
  author={Catherine R. Chesnutt and Lee Niswander},
  journal={Genesis},
  year={2004},
  volume={39 2},
  pages={73-8}
}
The chicken neural tube serves as an ideal model for new techniques to alter gene expression. We show that short-hairpin RNA delivered by a plasmid vector can effectively knock down expression of both exogenous and endogenous genes in the chicken neural tube. We also assay the effects of short-hairpin RNA on the interferon response and find no difference between electroporation of a control plasmid and a short-hairpin RNA plasmid. 

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