Plasmid-based one-pot saturation mutagenesis

@article{Wrenbeck2016PlasmidbasedOS,
  title={Plasmid-based one-pot saturation mutagenesis},
  author={Emily E Wrenbeck and Justin R Klesmith and James A. Stapleton and Adebola Adeniran and Keith E. J. Tyo and Timothy A Whitehead},
  journal={Nature Methods},
  year={2016},
  volume={13},
  pages={928-930}
}
Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, single-day, one-pot saturation mutagenesis method performed on routinely prepped… CONTINUE READING

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