Plasmid and chromosomal DNA recovery by electroextraction of cyanobacteria.

  title={Plasmid and chromosomal DNA recovery by electroextraction of cyanobacteria.},
  author={Dan Moser and Daniel G. Zarka and Curtis Hedman and Toivo Kallas},
  journal={FEMS microbiology letters},
  volume={128 3},
High voltage electroporation has been investigated as a method for rapid recovery of plasmid and chromosomal DNA from the cyanobacteria Nostoc PCC 7121, Synechococcus PCC 7002, and Anabaena PCC 7120. Pulses of 18 kV/cm and higher applied to concentrated Nostoc cells carrying a shuttle plasmid (pRL25) resulted in copious release of nucleic acids and phycobiliproteins into the suspending medium. Small portions of these supernatants, when electroporated with Escherichia coli, gave rise to hundreds… CONTINUE READING

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