Plasmid addiction genes of bacteriophage P1: doc, which causes cell death on curing of prophage, and phd, which prevents host death when prophage is retained.

@article{Lehnherr1993PlasmidAG,
  title={Plasmid addiction genes of bacteriophage P1: doc, which causes cell death on curing of prophage, and phd, which prevents host death when prophage is retained.},
  author={Hansj{\"o}rg Lehnherr and Emmanuelle Maguin and Samina Jafri and Michael B. Yarmolinsky},
  journal={Journal of molecular biology},
  year={1993},
  volume={233 3},
  pages={414-28}
}
P1 lysogens of Escherichia coli carry the prophage as a stable low copy number plasmid. The frequency with which viable cells cured of prophage are produced is about 10(-5) per cell per generation. Here we show that a significant part of this remarkable stability can be attributed to a plasmid-encoded mechanism that causes death of cells that have lost P1. In other words, the lysogenic cells appear to be addicted to the presence of the prophage. The plasmid withdrawal response depends on a gene… CONTINUE READING

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