Plasmid DNA supercoiling and gyrase activity in Escherichia coli wild-type and rpoS stationary-phase cells.

@article{ReyesDomnguez2003PlasmidDS,
  title={Plasmid DNA supercoiling and gyrase activity in Escherichia coli wild-type and rpoS stationary-phase cells.},
  author={Yazmid Reyes-Dom{\'i}nguez and Gabriel Contreras-Ferrat and Jes{\'u}s Ram{\'i}rez-Santos and Jorge Membrillo-Hern{\'a}ndez and M. Carmen G{\'o}mez-Eichelmann},
  journal={Journal of bacteriology},
  year={2003},
  volume={185 3},
  pages={1097-100}
}
Stationary-phase cells displayed a distribution of relaxed plasmids and had the ability to recover plasmid supercoiling as soon as nutrients became available. Preexisting gyrase molecules in these cells were responsible for this recovery. Stationary-phase rpoS cells showed a bimodal distribution of plasmids and failed to supercoil plasmids after the… CONTINUE READING