Plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (Pimephales promelas).

@article{Brodeur2006PlasmaSA,
  title={Plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (Pimephales promelas).},
  author={Julie C{\'e}line Brodeur and Kent B Woodburn and Fagen Zhang and Mike Bartels and Gary M. Kle{\vc}ka},
  journal={Environmental toxicology and chemistry},
  year={2006},
  volume={25 2},
  pages={337-48}
}
The present study compared three different methods for measuring plasma vitellogenin (VTG) in fathead minnow (FHM; Pimephales promelas): A procedure using liquid chromatography with electrospray ionization combined with tandem mass spectrometry (LC/ESI-MS/MS), and two commercial enzyme-linked immunoassay (ELISA) kits using either anti-carp or anti-FHM antibodies. The influence on plasma VTG measurements of using the protease-inhibitor aprotinin during blood sampling and of submitting the plasma… CONTINUE READING