Plasma membrane cholesterol: a possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolism.

@article{Khan2003PlasmaMC,
  title={Plasma membrane cholesterol: a possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolism.},
  author={Nadeem Akbar Khan and Jiangang Shen and Ta Yuan Chang and Catherine C Chang and Peter Chin Wan Fung and Oleg Y. Grinberg and Eugene Demidenko and Harold Swartz},
  journal={Biochemistry},
  year={2003},
  volume={42 1},
  pages={23-9}
}
The effect of the cholesterol content of the plasma membrane on the intracellular concentration of oxygen in Chinese hamster ovary (CHO) cells and their mutants was investigated by EPR oximetry. Total and free cholesterol content was significantly higher in 25 RA CHO cells as compared to wild-type and M 19 CHO cells, with most of the free cholesterol in normal and mutant CHO cells located in the plasma membrane. The plasma membrane cholesterol content also was altered by various biochemical… CONTINUE READING