Plasma levels and in vitro production of tumor necrosis factor-alpha and interleukin-6 in patients with amebic liver abscess.

@article{GonzlezAmaro1994PlasmaLA,
  title={Plasma levels and in vitro production of tumor necrosis factor-alpha and interleukin-6 in patients with amebic liver abscess.},
  author={Roberto Gonz{\'a}lez-Amaro and S. Sanchez Andrade and Lourdes Baranda and Carlos Abud-Mendoza and D P Portales and Benjam{\'i}n Moncada and Luis Llorente},
  journal={Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion},
  year={1994},
  volume={46 3},
  pages={209-13}
}
We studied the plasma levels of tumor necrosis factor-alpha (TNF-alpha) interleukin-6 (IL-6) as well as the in vitro production of these cytokines by peripheral blood mononuclear cells, in 10 patients with amebic liver abscess (ALA) and seven healthy controls. TNF-alpha was measured using a bioassay with L929 fibroblasts; IL-6 was quantitated by the B9 cell line assay. In both assays, the number of viable cells was estimated by the conversion of MTT to formazan. TNF-alpha plasma levels were… CONTINUE READING