Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA1 are resistant to virus infection.

@article{MacFarlane1992PlantsTW,
  title={Plants transformed with a region of the 201-kilodalton replicase gene from pea early browning virus RNA1 are resistant to virus infection.},
  author={Stuart A MacFarlane and Jeffrey W. Davies},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 13},
  pages={5829-33}
}
The 3' proximal portion of the gene encoding the 201-kDa putative replicase protein from the Tobravirus pea early browning virus (PEBV) can potentially be expressed separately as a 54-kDa protein. Nicotiana benthamiana plants transformed with the open reading frame (ORF) encoding the 54-kDa protein, designated 54K ORF, were resistant to infection by purified PEBV at inoculum doses of up to 1 mg/ml, the highest concentration tested. However, resistance was abolished by the introduction into the… CONTINUE READING

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