Plants transformed with a cistron of a potato virus Y protease (NIa) are resistant to virus infection.

@article{Vardi1993PlantsTW,
  title={Plants transformed with a cistron of a potato virus Y protease (NIa) are resistant to virus infection.},
  author={Eyal Vardi and Ilan Sela and Orit Edelbaum and Orna Livneh and L. Ya. Kuznetsova and Yehuda Stram},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 16},
  pages={7513-7}
}
An oligonucleotide carrying signals for translation initiation in plants was engineered upstream to a cDNA clone containing nucleotides 5812-7260 of the potato virus Y (PVY) genome. This fragment contains all but the first 100 5' terminal bases of the cistron encoding one of the PVY proteases (NIa) as well as the first 251 bases of the next cistron (NIb). Nicotiana tabacum cv. SR1 plants were transformed with this fragment. The presence of the NIa sequences in transformed plants was determined… CONTINUE READING