Plants combat infection by gene silencing

  title={Plants combat infection by gene silencing},
  author={Simon N. Covey and Nadia S. Al-Kaff and Amagoia L{\'a}ngara and David S. Turner},
Symptom recovery in virus-infected plants: Revisiting the role of RNA silencing mechanisms.
How a delicate equilibrium between RNA silencing and virus counter-defense responses in recovered leaves may help maintain virus titers at levels below the threshold required for symptom induction is discussed. Expand
Recovery from Cucurbit leaf crumple virus (family Geminiviridae, genus Begomovirus) infection is an adaptive antiviral response associated with changes in viral small RNAs.
Results suggest that recovery from CuLCrV infection is an adaptive antiviral defense mechanism, most likely mediated by gene silencing. Expand
Symptom Remission and Specific Resistance of Pepper Plants After Infection by Pepper golden mosaic virus.
Under controlled conditions for growth and inoculation with a low-pressure biolistic device, PepGMV-infected pepper plants consistently showed symptom remission or host recovery 12 to 15 days postinoculation, suggesting that a population of viral molecules escape from plant defensive mechanisms to maintain a subliminal, symptomless infection. Expand
Evidence for RNA-mediated defence effects on the accumulation of Potato leafroll virus.
The results support the suggestion that an RNA-mediated form of resistance that resembles post-transcriptional gene silencing operates in non-vascular cells and may be part of the mechanism that restricts PLRV to vascular tissue in conventionally infected plants. Expand
RNA Silencing and its Inhibition in transgenic Tobacco Plants
The reactions of the silencing suppressor-expressing transgenic plants to five different virus infections showed that these suppressors affected the accumulation and spread of these homologous and heterologous viruses differently in two Nicotiana species. Expand
Crowdsourced Identification of Potential Target Genes for CTV Induced Gene Silencing for Controlling the Citrus Greening Vector Diaphorina citri
A virus-induced gene silencing (VIGS) can be used to test future transgenic cultivars before genetically engineering citrus to identify transcriptionally modified genes located upstream and downstream of the targeted RIE gene. Expand
Harnessing model organism genomics to underpin the machine learning-based prediction of essential genes in eukaryotes - Biotechnological implications.
An historical review of experimental and computational approaches employed for the characterisation of essential genes in eukaryotes is undertaken, with a particular focus on model ecdysozoans (C. elegans and D. melanogaster), and the possible applicability of ML-approaches to organisms such as socioeconomically important parasites is discussed. Expand
Development of a Virus‐Induced Gene Silencing System for Dioecious Coccinia grandis
The results suggest that ALSV-VIGS is suitable for characterization of gene function in dioecious C. grandis and it can help to understand the mechanism of sex expression. Expand
Evolving Mechanisms of Disease Tolerance
Exploring Promises of siRNA in Cancer Therapeutics
This mini-review focuses on the potential of small interfering RNAs (siRNA) in anticancer treatment, and describes the significant barriers that exist on the path to clinical application of siRNA drugs. Expand


Ectopic pairing of homologous DNA and post-transcriptional gene silencing in transgenic plants
It is shown that co-suppression and other types of post-transcriptional gene silencing in plants are initiated by homology-dependent pairing of transgene sequences, and the silencing mechanism acts in the cytoplasm to prevent accumulation of all RNAs with homology to thetransgene. Expand
  • P. Meyer, H. Saedler
  • Biology, Medicine
  • Annual review of plant physiology and plant molecular biology
  • 1996
Different mechanistic models for plant-specific inactivation mechanisms and their relationship with repeat-specific silencing phenomena in other species are discussed. Expand
Mechanisms of Pathogen-Derived Resistance to Viruses in Transgenic Plants.
The major emphasis is on the mechanisms of pathogen-derived resistance rather than on the practicality of using this technology for crop improvement, because these mechanisms have proven difficult to unravel. Expand
Biological diversity of cauliflower mosaic virus isolates expressed in two Brassica species
No clear correlation between virus titre and symptom type and severity in turnip is observed, although plants infected with viral isolates producing mild symptoms generally, but not always, contained less viral DNA than isolates causing more severe symptoms. Expand
How and Why Do Plants Inactivate Homologous (Trans)genes?
Gene silencing in transgenic plants has emerged in the last 5 years as a topic of intense interest for both applied and basic plant scientists and a small cadre of basic scientists has become fascinated by the phenomenon and is analyzing a variety of silencing systems. Expand
Inactivation of gene expression in plants as a consequence of specific sequence duplication.
  • R. Flavell
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1994
It is suggested that the mechanisms evolved to help silence the many copies of transposable elements in plants must have evolved to avoid these silencing mechanisms or their consequences. Expand
Pararetroviruses and retroviruses: a comparative review of viral structure and gene expression strategies.
Publisher Summary This chapter addresses some aspects of the gene expression strategies and viral structure of retroviruses and pararetroviruses, highlighting the common nature of the problems facedExpand
Host regulation of the cauliflower mosaic virus multiplication cycle.
It is concluded that expression of the CaMV minichromosome is a key phase of the virus multiplication cycle, which is regulated differentially in organs of a highly susceptible host species and controls host susceptibility toCaMV infection. Expand