Plant virus expressing hybrid coat protein with added murine epitope elicits autoantibody response.

@article{Fitchen1995PlantVE,
  title={Plant virus expressing hybrid coat protein with added murine epitope elicits autoantibody response.},
  author={John Fitchen and Roger N. Beachy and Mich B. Hein},
  journal={Vaccine},
  year={1995},
  volume={13 12},
  pages={1051-7}
}
A modified tobacco mosaic virus (TMV) was used to direct the synthesis of TMV coat protein hybrids containing a 13 amino acid sequence of the murine zona pellucida ZP3 protein. This hybrid protein was found to be robust and accumulate to high concentrations in inoculated plants. Virus-like particles containing the hybrid coat protein could be readily purified from infected plant tissue. Parenteral immunization with these virus-like particles as antigen resulted in serum antibody recognizing ZP3… CONTINUE READING