Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.)

@article{Firoozabady2007PlantRV,
  title={Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.)},
  author={Ebrahim Firoozabady and David L. DeBoer},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2007},
  volume={29},
  pages={166-173}
}
Embryogenic callus was formed from several cultivars of cotton (Gossypium hirsutum L.) when sections of hypocotyl and cotyledon were cultured on medium supplemented with 5 mg/liter 6-(γ, γ-dimethylallyl-amino)-purine (2iP) and 0.1 mg/liter α-naphthaleneacetic acid (NAA) for callus initiation and proliferation, and subcultured on medium supplemented with 5 mg/liter NAA and 0.1 to 1 mg/liter 2iP for embryogenic callus induction. It seems that a high 2iP:auxin ratio is preferred for callus… CONTINUE READING

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Rapid plant regeneration from Nicotiana mesophyll protoplasm

E. Firoozabady
Plant Sci • 1986

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