Plant regeneration from mesophyll protoplasts of Moricandia arvensis

Abstract

Moricandia arvensis is of interest as it is a dicotyledonous species which has C3 — C4 intermediate photosynthesis, a mechanism which results in enhanced recapture of photorespired CO2. Leaves from cultured shoot tips were used as a source for mesophyll cell protoplasts. Approximately 1% of the protoplasts which survived the first few days of culture produced calli. On a suitable regeneration medium, 30–60% of the calli regenerated one or more shoots. From among the regenerating shoots eight were selected, transferred to soil and grown to flowering in the glasshouse; all were fertile. The development of a protoplast regeneration system provides the opportunity to use transformation and somaclonal variation as tools in the genetic analysis of the C3–C4 character in this species.

DOI: 10.1007/BF00234371

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Cite this paper

@article{Murata1992PlantRF, title={Plant regeneration from mesophyll protoplasts of Moricandia arvensis}, author={Tasuku Murata and Raymond J. Mathias}, journal={Plant Cell Reports}, year={1992}, volume={11}, pages={408-411} }