Plant regeneration from isolated microspore cultures of Chinese cabbage (Brassica campestris spp. pekinensis)

Abstract

Isolated microspores of Chinese cabbage (Brassica campestris ssp. pekinensis) were incubated in modified NN medium containing 10% sucrose in darkness at 33°C for one day followed by culture at 25°C. After 14 days of culture, microspores developed into embryos ranging from globular to cotyledonary stage. Plants were regenerated after transfer of embryos to medium containing 3% sucrose and no plant growth regulators.

DOI: 10.1007/BF00269055

Cite this paper

@article{Sato1989PlantRF, title={Plant regeneration from isolated microspore cultures of Chinese cabbage (Brassica campestris spp. pekinensis)}, author={Takanori Sato and Takeshi Nishio and Masashi Hirai}, journal={Plant Cell Reports}, year={1989}, volume={8}, pages={486-488} }