Plant regeneration from callus protoplasts of the forage legume Astragalus adsurgens Pall.

  title={Plant regeneration from callus protoplasts of the forage legume Astragalus adsurgens Pall.},
  author={J Luo and J.-F. Jia},
  journal={Plant Cell Reports},
A reproducible release of viable protoplasts was obtained from friable calli of Astragalus adsurgens. Protoplasts underwent sustained divisions and formed cell colonies when cultured in either liquid or agarose-solidified Kao and Michayluk (1975) protoplast medium (KM8P) supplemented with 1.5 mg/l 2,4-D, 0.5 mg/l BA and 0.5 M glucose. Compared to liquid culture, agarose bead culture improved division frequency effectively, the two culture systems showing a plating efficiency of 0.8±0.5% and 6.5… CONTINUE READING
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