Plant regeneration from Ilex spp. (Aquifoliaceae) in vitro.

@article{Sansberro2001PlantRF,
  title={Plant regeneration from Ilex spp. (Aquifoliaceae) in vitro.},
  author={Pedro Alfonso Sansberro and Hebe Yolanda Rey and Luis Amado Mroginski and M A Krivenki},
  journal={Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al},
  year={2001},
  volume={25 2},
  pages={139-46}
}
In vitro plant regeneration from nodal segments (containing one axillary bud) of seven species of the genus Ilex (I. argentina, I. brevicuspis, I. dumosa, I. microdonta, I. pseudoboxus, I. taubertiana and I. theezans) were readily achieved through three steps: 1) shoot regeneration by in vitro culture of nodal segments in MS medium at 1/4 strength, plus 3% sucrose and 0.65% agar (1/4MS) and 0.5 microM BA (45 days of culture); 2) Induction of rooting from regenerated shoots with 1/4MS… CONTINUE READING