Plant regeneration from Gossypium davidsonii protoplasts via somatic embryogenesis

  title={Plant regeneration from Gossypium davidsonii protoplasts via somatic embryogenesis},
  author={Xingyu Yang and Xiaoping Guo and Xianlong Zhang and Yichun Nie and Shuangxia Jin},
  journal={Biologia Plantarum},
Protoplasts isolated from wild cotton Gossypium davidsonii were cultured in KM8P medium supplemented with different phytohormones. The most effective combination was 0.45 µM 2,4-dichlorophenoxyacetic acid, 2.68 µM α-naphthaleneacetic acid and 0.93 µM kinetin and the division percentage at the 8th day was 30.78 ± 3.04 %. The density of protoplasts at 2–10 × 105 cm−3 was suitable for protoplast division and calli formation, with a division percentage of 32.21 ± 3.64 % and a plating efficiency of… CONTINUE READING


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A revised medium for rapid grouth and bioassay with tobacco tissue cultures

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