Plant regeneration and micropropagation of Brachypodium distachyon

  title={Plant regeneration and micropropagation of Brachypodium distachyon},
  author={Pauline Bablak and John Draper and Michael Raymond Davey and Paul T. Lynch},
  journal={Plant Cell, Tissue and Organ Culture},
Brachypodium distachyon (L.) P. Beauv. has several features of its genome and growth habit reminiscent of Arabidopsis thaliana (L.) Heyn. that may allow it to be developed as a model molecular genetic system representative of the temperate grasses. In order for B. distachyon to be exploited in this way, it will be necessary to develop tissue culture procedures. This report details initial studies of the characteristics of mature seed-derived callus and the production of fertile plants from… 
Callus induction and plant regeneration from immature embryos of Brachypodium distachyon with different chromosome numbers
The paper reports the in vitro cultivation of two commercial lines and 23 wild populations of Brachypodium distachyon, and supports the idea that the three cytotypes of Brachymodium cultured actually belong to different species.
Optimizing callus induction and proliferation for Agrobacterium-mediated transformation of Brachypodium distachyon
Results showed that 2.5 mg l−1 2,4-D is an optimal concentration to use for both callus induction and proliferation, although 5.0 mg l+1 may also be used for callus proliferation, and results indicated that hygromycin is significantly more efficient than bialaphos when using the Agrobacterium-mediated transformation system.
One-Week Scutellar Somatic Embryogenesis in the Monocot Brachypodium distachyon
The fast SE induction method described here facilitates the mechanistic study of the processes involved and may significantly shorten the production of transgenic or gene-edited plants.
High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3
The identification of a transformable inbred diploid line, Bd21-3, and the development of a transformation method with transformation efficiencies as high as 41% of co-cultivated calluses producing transgenic plants are described.
Agrobacterium-mediated transformation of the temperate grass Brachypodium distachyon (genotype Bd21) for T-DNA insertional mutagenesis.
A facile, efficient and rapid transformation system for Bd21 is described using Agrobacterium-mediated transformation of compact embryogenic calli derived from immature embryos to facilitate large-scale functional genomics research in this model system.
A rapid and efficient transformation protocol for the grass Brachypodium distachyon
A fast and efficient microprojectile bombardment-mediated transformation protocol is reported for the grass species Brachypodium distachyon, a proposed alternative model plant to Oryza sativa for
Brachypodium distachyon, a New Model for the Triticeae
This chapter provides an overview of the advantages of Brachypodium as a model system and surveys the use and potential applications of this system to aid wheat, barley and Lolium research.
Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon
The development of inbred lines from 27 accessions of Brachypodium are described and it is indicated that most of the lines contained an insertion at a single genetic locus.
A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21
An improved procedure is provided for the facile and efficient Agrobacterium-mediated transformation of line Bd21, a novel model system for structural and functional genomics studies of temperate grasses because of its biological and genetic attributes.


Tissue culture and plant regeneration from immature embryo explants of Barley, Hordeum vulgare
Immature embryo explants taken 8 days after anthesis were used to establish callus cultures of spring barley, finding Gamborg's B5 basal medium supplemented with either 2,4-D or Cl3 POP to give good callus growth and shoot initiation and Regeneration of plantlets.
Somatic embryogenesis in sugarcane (Saccharum officinarum L.) I. The morphology and physiology of callus formation and the ontogeny of somatic embryos
The embryogenic callus was formed by divisions in mesophyll cells situated primarily in the abaxial half of the leaf, and also from cells of the vascular parenchyma, and showed the typical organization of grass embryos.
In vitro shoot culture of wild Oryzae and other grass species
It is envisaged that this clonal propagation technique could aid the genetic manipulation of cultivated rice by providing a means to vegetatively conserve valuable genetic resources, a technique to rapidly multiply novel hybrid material and a source of embryogenic callus that will allow the application of biotechnological techniques, such as somatic hybridization and genetic transformation, to previously unexploited species.
Rapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum)
A protocol for transformation of a highly embryogenic wheat cultivar, Bobwhite, revealing stable transformation was confirmed by marker gene expression assays and the presence of the bar sequences in high molecular weight chromosomal DNA of the resultant plants.
Cell culture of the Poaceae (Gramineae)
Plant cell and tissue culture constitutes an important and integral part of modern plant biotechnology including in the areas of application such as mass propagation, mass production of chemicals,
Induction of embryogenic Triticum aestivum L. calli. I. Quantification of genotype and culture medium effects
Somatic embryo (embryoid) formation from immature-embryo-derived calli was quantified in replicated experiments involving 10 Triticum aestivum L. genotypes and there were highly significant differences among genotypes, media, and individual plants from which explants were taken.
Plant regeneration in vitro from embryogenic cultures of spring- and winter-type barley (Hordeum vulgare L.) varieties
Experiments with field grown material in 3 consecutive years showed that environmental factors also strongly influenced the induction of somatic embryogenesis and plant regeneration.
Induction of haploidy from pollen grains in angiosperms — the current status
This review has made an effort to critically examine existing reports with the objective of analysing the effects of various factors and speculate on the mechanism of action of different factors in order to throw some light on the process of haploid induction.
Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.)
Callus was initiated from mature embryos of the Japonica cultivar Taipei 309 of rice to obtain a reproducible efficient procedure for regeneration of rice plants through somatic embryogenesis from callus and was found to be the most efficient.