Plant mitosis promoting factor disassembles the microtubule preprophase band and accelerates prophase progression in Tradescantia.

@article{Hush1996PlantMP,
  title={Plant mitosis promoting factor disassembles the microtubule preprophase band and accelerates prophase progression in Tradescantia.},
  author={J Hush and Liping Wu and Peter Crook Lloyd John and Loren H. Hepler and Peter K Hepler},
  journal={Cell biology international},
  year={1996},
  volume={20 4},
  pages={
          275-87
        }
}
The regulation of mitosis in higher plant cells has been investigated by microinjecting protein kinase from the metaphase-arresting (met1) mutant of Chlamydomonas. Biochemical characterization of this enzyme complex confirms the presence of a p34cdc2/cyclin B-like kinase. The enzyme was injected into living stamen hair cells of Tradescantia virginiana in which microtubules (MTs) were visualized using fluorescent analogue cytochemistry and confocal laser scanning microscopy. Microinjection of… CONTINUE READING
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