Plant microbody proteins, III. Labelling of the peroxisomal membrane protein SP-63 in vitro and in vivo.

@article{Ludwig1976PlantMP,
  title={Plant microbody proteins, III. Labelling of the peroxisomal membrane protein SP-63 in vitro and in vivo.},
  author={Bottenbruch Ludwig and Helmut Kindl},
  journal={Hoppe-Seyler's Zeitschrift fur physiologische Chemie},
  year={1976},
  volume={357 3},
  pages={
          393-9
        }
}
Methods were developed to charcterize membranes and membrane components of leaf peroxisomes from Lens culinaris. While microbodies from etiolated young leaves exhibited an equilibrium density of 1.19 g/cm3, older leaves or leaves exposed to light for increasing periods of time contained microbodies banding at higher densities up to 1.235 g/cm3. Similar values were also found for the corresponding microbody membranes, which could be labelled with diazotized [35S]sulphanilic acid. Labelling was… CONTINUE READING