Plant holo-(acyl carrier protein) synthase.

  title={Plant holo-(acyl carrier protein) synthase.},
  author={S A Elhussein and J{\'a}n A. Miernyk and John B. Ohlrogge},
  journal={The Biochemical journal},
  volume={252 1},
1. An improved method was developed for the assay of plant holo-(acyl carrier protein) synthase activity, using Escherichia coli acyl-(acyl carrier protein) synthetase as a coupling enzyme. 2. Holo-(acyl carrier protein) synthase was partially purified from spinach (Spinacia oleracea) leaves by a combination of (NH4)2SO4 fractionation and anion-exchange and gel-permeation chromatography. 3. The partially purified enzyme had a pH optimum of 8.2 and Km values of 2 microM, 72 microM and 3 mM for… CONTINUE READING

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