Plant development in long-term embryogenic callus lines of Cucurbita pepo

@article{Jureti1991PlantDI,
  title={Plant development in long-term embryogenic callus lines of Cucurbita pepo},
  author={Biserka Jureti{\'c} and Sibila Jelaska},
  journal={Plant Cell Reports},
  year={1991},
  volume={9},
  pages={623-626}
}
Embryogenic callus derived from pumpkin hypocotyl segments was induced and maintained for 15 years on MS medium supplemented with the auxins IBA (4.9 μM), 2, 4-D (4.5 μM) or IAA (5.7 μM). On induction media continued embryo maturation and development of adult plants typically failed. Therefore, small embryogenic clumps and individually isolated embryos were subcultured two to four times on one of the conversion media: MS supplemented with 1.5% sucrose and (a) no hormone, (b) 2.9 μM IAA, (c) 5.7… CONTINUE READING