Plant cholesterol biosynthetic pathway overlaps with phytosterol metabolism

@article{Sonawane2016PlantCB,
  title={Plant cholesterol biosynthetic pathway overlaps with phytosterol metabolism},
  author={Prashant D. Sonawane and Jacob Pollier and Sayantan Panda and Jędrzej Szymański and Hassan Massalha and Meital Yona and Tamar Unger and Sergey Malitsky and Philipp Arendt and Laurens Pauwels and Efrat Almekias-Siegl and Ilana Rogachev and Sagit Meir and Pablo D C{\'a}rdenas and Athar Masri and Marina Petrikov and Hubert Schaller and Arthur A. Schaffer and Avinash C Kamble and A. Giri and Alain Goossens and Asaph Aharoni},
  journal={Nature Plants},
  year={2016},
  volume={3}
}
The amount of cholesterol made by many plants is not negligible. [...] Key Result Integrating tomato transcript and protein co-expression data revealed candidate genes putatively associated with cholesterol biosynthesis. A combination of functional assays including gene silencing, examination of recombinant enzyme activity and yeast mutant complementation suggests the cholesterol pathway comprises 12 enzymes acting in 10 steps.Expand
Dissecting cholesterol and phytosterol biosynthesis via mutants and inhibitors.
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The complexity and variation of the biosynthetic routes of the most abundant phytosterols and cholesterol in the green lineage are reviewed and how different enzymes in these pathways are conserved and diverged from humans, yeast and even bacteria are reviewed. Expand
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This work resolves the technical bottlenecks in phytosterol-derived pathway reconstitution in the backer’s yeast and opens up opportunities for efficient bioproduction and pathway elucidation of this group of phytochemicals. Expand
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The use of mutants and inhibitors to study sterol biosynthesis in plants
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The complexity and variation of the biosynthetic routes of the most abundant phytosterols in the green lineage are reviewed and how different enzymes in these pathways are conserved and diverged from humans,yeast and even bacteria are discussed. Expand
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A complex, non-uniform history for the loss of anthocyanin production across betalain producers is implied, previously assumed to be strictly due to diminished expression of antho-related genes. Expand
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The breeding of high CoQ10 tomato lines is reported by expressing four enzymes with a fruit-specific promoter, which modifies the chloroplast chorismate pathway, enhances cytosolic isoprenoid biosynthesis, and up-regulates the first two reactions in mitochondrion that construct the CoQ 10 polyisoprenoids tail. Expand
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