Plant cholesterol biosynthetic pathway overlaps with phytosterol metabolism

@article{Sonawane2016PlantCB,
  title={Plant cholesterol biosynthetic pathway overlaps with phytosterol metabolism},
  author={P. Sonawane and Jacob Pollier and S. Panda and J. Szymański and H. Massalha and Meital Yona and T. Unger and S. Malitsky and P. Arendt and L. Pauwels and Efrat Almekias-Siegl and I. Rogachev and S. Meir and P. D. C{\'a}rdenas and A. Masri and M. Petrikov and H. Schaller and A. Schaffer and A. Kamble and A. Giri and A. Goossens and A. Aharoni},
  journal={Nature Plants},
  year={2016},
  volume={3}
}
The amount of cholesterol made by many plants is not negligible. [...] Key Result Integrating tomato transcript and protein co-expression data revealed candidate genes putatively associated with cholesterol biosynthesis. A combination of functional assays including gene silencing, examination of recombinant enzyme activity and yeast mutant complementation suggests the cholesterol pathway comprises 12 enzymes acting in 10 steps.Expand
Dissecting cholesterol and phytosterol biosynthesis via mutants and inhibitors.
TLDR
The complexity and variation of the biosynthetic routes of the most abundant phytosterols and cholesterol in the green lineage are reviewed and how different enzymes in these pathways are conserved and diverged from humans, yeast and even bacteria are reviewed. Expand
Engineering of Phytosterol-Producing Yeast Platforms for Functional Reconstitution of Downstream Biosynthetic Pathways
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This work resolves the technical bottlenecks in phytosterol-derived pathway reconstitution in the backer’s yeast and opens up opportunities for efficient bioproduction and pathway elucidation of this group of phytochemicals. Expand
Engineering of Phytosterol-Producing Yeast Platforms for Functional Reconstitution of Downstream Biosynthetic Pathways.
TLDR
This work identified and addressed some of the technical bottlenecks in phytosterol-derived pathway reconstitution in the baker's yeast and opens up opportunities for efficient bioproduction and metabolic pathway elucidation of this group of phytochemicals. Expand
Short-chain dehydrogenase/reductase governs steroidal specialized metabolites structural diversity and toxicity in the genus Solanum
TLDR
It is found that the tomato GLYCOALKALOID METABOLISM25 (GAME25), a short-chain dehydrogenase/reductase, catalyzes the first of three prospective reactions required to reduce the C-5,6 double bond in dehydrotomatidine to form tomatidine. Expand
The use of mutants and inhibitors to study sterol biosynthesis in plants
TLDR
The complexity and variation of the biosynthetic routes of the most abundant phytosterols in the green lineage are reviewed and how different enzymes in these pathways are conserved and diverged from humans,yeast and even bacteria are discussed. Expand
THE BIOSYNTHESIS REGULATION OF POTATO STEROIDAL GLYCOALKALOIDS
TLDR
The purpose of this review is to summarize the information on the known SGA biosynthesis genes in plants and the results of the investigation of these genes in potato, as well as to consider the mechanisms of the SGA protective toxic action against pathogens and pests. Expand
Multi‐omics data‐driven investigations of metabolic diversity of plant triterpenoids
TLDR
Gene mining through large-scale omics data and functional characterization of metabolic genes including enzymes, transcription factors and transporters could provide important insights into the evolution of specialized plant metabolism and pave the way for the production of high-value metabolites or derivatives using synthetic biology approaches. Expand
Plant Metabolic Gene Clusters: Evolution, Organization, and Their Applications in Synthetic Biology
Plants are a remarkable source of high-value specialized metabolites having significant physiological and ecological functions. Genes responsible for synthesizing specialized metabolites are oftenExpand
Transcriptome and Metabolic Profiling Provides Insights into Betalain Biosynthesis and Evolution in Mirabilis jalapa.
TLDR
A complex, non-uniform history for the loss of anthocyanin production across betalain producers is implied, previously assumed to be strictly due to diminished expression of antho-related genes. Expand
Diatoms synthesize sterols by inclusion of animal and fungal genes in the plant pathway
TLDR
The first elucidation of the complete biosynthesis of sterols in the lineage is reported, with a good level of similarity between the sterol biosynthetic genes of S. marinoi and C. cryptica with those in the genomes of the other diatoms sequenced so far. Expand
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