Plant chitinases use two different hydrolytic mechanisms.

  title={Plant chitinases use two different hydrolytic mechanisms.},
  author={Beatrice Iseli and Sylvie Armand and Thomas Boller and J M Neuhaus and Bernard Henrissat},
  journal={FEBS letters},
  volume={382 1-2},
Bacterial, fungal, animal, and some plant chitinases form family 18 of glycosyl hydrolases. Most plant chitinases form the family 19. While some chitinases also have lysozyme activity, animal lysozymes belong to different families. For glycosyl hydrolases, two reaction mechanisms are possible, leading to either retention or inversion of the anomeric configuration. We analyzed by HPLC the stereochemical outcome of the hydrolysis catalyzed by cucumber and bean chitinases, belonging to families 18… CONTINUE READING

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