Plant cell suspension culture in a bench-scale fermenter with a newly designed membrane stirrer for bubble-free aeration

Abstract

In this paper, tests of an optimized membrane-stirrer geometry for bubble-free aeration of a plant cell suspension culture are described. Cell attachment and clogging of a previously described system [Piehl et al. (1988) Appl Microbiol Biotechnol 29:456–461] led to the development of a new stirrer. The volumetric oxygen transfer capacity has been measured in aqueous medium. The mass transfer coefficient, k l a, was 3.75 h−1 at 25 °C and at a stirrer speed of 34 rpm. The overall oxygen transfer capacity was investigated with a suspension culture of Aesculus hippocastanum. It was shown that the oxygen mass transfer was sufficient even at the maximum biomass of 10–12 g dry weight/l, which was obtained by using this system. Furthermore, special attention was given to medium components like C and N sources, to avoid growth limitation due to a shortage of nutrients.

DOI: 10.1007/s002530051030

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@article{Boehme1997PlantCS, title={Plant cell suspension culture in a bench-scale fermenter with a newly designed membrane stirrer for bubble-free aeration}, author={Christoph Boehme and M. Schroeder and H. Jung-Heiliger and J{\"{u}rgen Lehmann}, journal={Applied Microbiology and Biotechnology}, year={1997}, volume={48}, pages={149-154} }