Plant Regeneration from Protoplasts of Lycopersicon esculentum Mill.

  title={Plant Regeneration from Protoplasts of Lycopersicon esculentum Mill.},
  author={Alison Morgan and Edward C. Cocking},
  journal={Zeitschrift f{\"u}r Pflanzenphysiologie},

Culture and regeneration of Lycopersicon peruvianum leaf protoplasts

It is suggested that the effectiveness of medium for protoplast culture depends on endogenous conditions of the plant before the isolation of protoplasts, and that calluses with a shorter period of culture were suitable for achieving a higher rate of normal shoot formation with abundant shoots per callus.

Lycopersicon Esculentum: Globular Embryos from Microspores and Calli from Diploid Protoplasts

The goal of this work was to develop culture conditions which allow subsequent division of isolated tomato microspores to built up a protoplast regeneration system in order to extend the list of tomato cultivars, from which protoplasts-derived calli or plants can be obtained.

Plant regeneration from leaf protoplasts of Lycopersicon hirsutum f. hirsutum

A protocol is described for high frequency plant regeneration from isolated leaf protoplasts of several genotypes of the wild tomato species Lycopersicon hirsutum f. hirsutum based on modified tomato

Protoplast and leaf explant culture of Lycopersicon cheesmanii and salt tolerance of protoplast-derived calli

In vitro manipulation of Lycopersicon cheesmanii was attempted in order to evaluate its potential to improve salt tolerance of L. esculentum following somatic hybridisation of these two species. The

Somatic hybridization between Lycopersicon esculentum and Lycopersicon pennellii

This result suggests that the non-regenerable growth habit of the L. pennellii suspension culture was dominant in the somatic hybrid, and the culture conditions described here are suitable for obtaining regenerated plants from L. esculentum mesophyll protoplasts.

Plant regeneration from protoplasts isolated from cell suspension cultures of the wild tomato, Lycopersicon chilense Dun.

A protocol has been established for rapid, high frequency plant regeneration from protoplasts of the wild tomato species Lycopersicon chilense Dun, and up to 70% of protoplast-derived calli regenerated shoots when cultured on agar-solidified medium.

Plant regeneration from mesophyll protoplasts of tomato cv. Ponderosa

Axenic seedlings of tomato cv. Ponderosa were used as material for protoplast culture. Mesophyll protoplasts were treated in an enzyme solution containing 0.5% Cellulase“Onozuka”RS and 0.1%

A genetic analysis of cell culture traits in tomato

SummaryTomato genotypes superior in regenerating plants from protoplast and callus cultures were obtained by transferring regeneration capacity from Lycopersicon peruvianum into L. esculentum by



Plantlet formation from Lycopersicon esculentum leaf callus

Explants were obtained from leaves of three strains of tomato and grown on a modified Murashige and Skoog's medium with various combinations of indole-3-acetic acid (IAA) and kinetin and shoot formation occurred within 30 days but only at a specific combination of concentrations of the two growth hormones.

Morphogenetic Potential of Cultured Leaf Sections of Cultivated and Wild Species of Tomato

The wild species Lp, was found to have the highest morphogenetic potential as expressed by root and shoot formation in the various media, as compared with the other species.

The Effect of 2,3.5 Triiodobenzoic Acid on Caulogenesis in Callus Cultures of Tomato and Pelargonium

It is suggested that auxin synthesis may occur at some microscopic stage in morphogenesis in ‘geranium’ cultures which suppresses further caulogenesis, which may be overcome by the addition of TIBA to the medium at the appropriate stage in Morphogenesis.