Plant Regeneration from Mesophyll Protoplasts of Rice (Oryza sativa L.)

  title={Plant Regeneration from Mesophyll Protoplasts of Rice (Oryza sativa L.)},
  author={Hari Shanker Gupta and Arunava Pattanayak},
We report induction of sustained divisions, colony formation and plant regeneration from mesophyll protoplasts isolated from the leaf base and sheath of rice seedlings. Protoplasts were purified on sucrose density gradients and cultured in modified liquid N6 medium in the presence of a feeder layer prepared from young rice-cell suspension cultures. Protoplasts formed cell walls and divided after 6 to 7 days giving rise to microcolonies which became macroscopic after 25 to 30 days. Nine to ten… CONTINUE READING
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