Plant Insecticide L-Canavanine Repels Drosophila via the Insect Orphan GPCR DmX

@article{Mitri2009PlantIL,
  title={Plant Insecticide L-Canavanine Repels Drosophila via the Insect Orphan GPCR DmX},
  author={Christian Mitri and Laurent Soustelle and B{\'e}r{\'e}nice Framery and Jo{\"e}l Bockaert and M L Parmentier and Yves Grau},
  journal={PLoS Biology},
  year={2009},
  volume={7}
}
For all animals, the taste sense is crucial to detect and avoid ingesting toxic molecules. Many toxins are synthesized by plants as a defense mechanism against insect predation. One example of such a natural toxic molecule is L-canavanine, a nonprotein amino acid found in the seeds of many legumes. Whether and how insects are informed that some plants contain L-canavanine remains to be elucidated. In insects, the taste sense relies on gustatory receptors forming the gustatory receptor (Gr… Expand
Gαo Is Required for L-Canavanine Detection in Drosophila
TLDR
This study revealed that Gαo47A plays a crucial role in L-canavanine detection, a variant G-protein coupled receptor belonging to the metabotropic glutamate receptor subfamily that is expressed in bitter-sensitive taste neurons of Drosophila. Expand
Gustatory Receptors Required for Avoiding the Insecticide l-Canavanine
TLDR
It is shown that GRs are essential for responding to l-canavanine and that flies missing DmXR displayed normal l-Canavanine avoidance and l- canavanine-evoked action potentials, and that GR8a contributes to the specificity for l- Canavanine. Expand
Multiple channels of DEET repellency in Drosophila.
TLDR
The findings support a multimodal mechanism for DEET detection in fruit flies and indicate a prominent role for taste detection mediating DEET repellence in Drosophila. Expand
Evolution and functional characterisation of the IR chemosensory receptors in the Drosophila larval gustatory system
TLDR
In contrast to the olfactory IRs that are well-conserved, gustatory IRs are rapidly evolving species-specific receptors that are likely to be involved in detecting a wide variety of tastants. Expand
l-Canavanine: How does a simple non-protein amino acid inhibit cellular function in a diverse living system?
TLDR
In higher plants, CAN has recently been used as a tool to study the regulation or modulation of polyamine–NO cross-talk, and seems to have the highest toxic properties. Expand
Molecular and Cellular Designs of Insect Taste Receptor System
TLDR
Analysis of the primary projection of taste neurons to the brain shows that the projection profiles depend basically on the peripheral locations of the neurons as well as the GRs that they express, suggesting that both peripheral and central design principles of insect taste perception are different from those of olfactory perception. Expand
Dual Mechanism for Bitter Avoidance in Drosophila
TLDR
It is postulate that sugar-sensing inhibition represents a mechanism in insects to prevent ingesting harmful substances occurring within mixtures and that it affects taste cells on the proboscis and the legs. Expand
G Protein-Coupled Receptors as Target Sites for Insecticide Discovery
TLDR
Diverse functions of insect GPCRs in feeding behavior, locomotion activity, metabolism, circadian rhythm, development and reproduction have been characterized in the past few years and possible GPCR targets and screening assays to identify insecticides that target GPCRS are discussed. Expand
A conserved odorant binding protein is required for essential amino acid detection in Drosophila
TLDR
It is demonstrated that fruit flies need an odorant-binding protein OBP19b, which is highly expressed in taste sensilla, to prefer select amino acids such as essential l-phenylalanine, to ensure their dietary intake of essential amino acids. Expand
Identification and comparative analysis of G protein-coupled receptors in Pediculus humanus humanus.
TLDR
The results indicated that the repertoire of GPCRs in P. h. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 63 REFERENCES
Multiple gustatory receptors required for the caffeine response in Drosophila
TLDR
It is proposed that Drosophila taste receptors that function in avoidance of bitter compounds are more complex and require additional subunits that remain to be identified. Expand
Taste and pheromone perception in the fruit fly Drosophila melanogaster
TLDR
The anatomical properties of the Drosophila gustatory system are described and the family of taste receptors are surveyed to provide an appropriate background and an outlook of taste research in this elegant model system for the next few years is provided. Expand
l-Canavanine: a higher plant insecticidal allelochemical
TLDR
Protein-based sequestration of canavanine prevented turnover and clearance of the free amino acid, and undoubtedly contributed significantly to the antimetabolic character of this protective allelochemical. Expand
Gustatory Perception and Behavior in Drosophila melanogaster
TLDR
An update on progress in understanding taste perception in the model fruit fly Drosophila melanogaster is presented, and three specific proposals that seem uniquely applicable to this exquisite model system and cannot, at least currently, be pursued elsewhere are provided. Expand
Gsα Is Involved in Sugar Perception in Drosophila melanogaster
TLDR
It is suggested that sugar-taste signals are processed through the Gsα-mediating signal transduction pathway in sugar-sensitive GRNs in Drosophila. Expand
Taste Perception and Coding in Drosophila
BACKGROUND Discrimination between edible and contaminated foods is crucial for the survival of animals. In Drosophila, a family of gustatory receptors (GRs) expressed in taste neurons is thought toExpand
L-Canavanine and protein synthesis in the tobacco hornworm Manduca sexta.
  • G. Rosenthal, D. Dahlman
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1986
L-Canavanine, a nonprotein amino acid of certain leguminous plants, manifests potent insecticidal properties in a canavanine-sensitive insect such as the tobacco hornworm Manduca sexta (L.)Expand
Molecular evolution of the insect chemoreceptor gene superfamily in Drosophila melanogaster
The insect chemoreceptor superfamily in Drosophila melanogaster is predicted to consist of 62 odorant receptor (Or) and 68 gustatory receptor (Gr) proteins, encoded by families of 60 Or and 60 GrExpand
Evolution, structure, and activation mechanism of family 3/C G-protein-coupled receptors.
TLDR
The recent resolution of the structure of this binding domain in one of these receptors, the metabotropic glutamate 1 receptor, revealed a unique mechanism of activation for these GPCRs, which open new possibilities in the development of drugs aimed at modulating these receptors. Expand
Drosophila Gr5a encodes a taste receptor tuned to trehalose
TLDR
It is demonstrated by heterologous expression in a Drosophila S2 cell line that Gr5a encodes a receptor tuned to trehalose, the first functional expression of an invertebrate taste receptor. Expand
...
1
2
3
4
5
...