Place of human amniotic membrane immunoblotting in the diagnosis of autoimmune bullous dermatoses.

@article{GrootenboerMignot2010PlaceOH,
  title={Place of human amniotic membrane immunoblotting in the diagnosis of autoimmune bullous dermatoses.},
  author={Sabine Grootenboer-Mignot and V. Descamps and Catherine Picard-Dahan and Pascale Nicaise-Roland and C Prost-Squarcioni and C Leroux-Villet and C Champagnat and Anne Delaval and Françoise Aucouturier and B{\'e}atrice Crickx and Sylvie Chollet-Martin},
  journal={The British journal of dermatology},
  year={2010},
  volume={162 4},
  pages={743-50}
}
BACKGROUND Fine analysis of antiskin autoantibodies can contribute to the differential diagnosis of autoimmune bullous dermatoses. OBJECTIVES To develop a high-performance immunoblotting method using human amniotic membrane as the antigen source, and to compare it with current laboratory methods. METHODS Sera from 113 patients were tested by immunoblotting (IB), rat and monkey oesophagus and salt-split skin indirect immunofluorescence (IIF), and enzyme-linked immunosorbent assay (ELISA… CONTINUE READING

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