Pinnacle: a fast, automatic and accurate method for detecting and quantifying protein spots in 2-dimensional gel electrophoresis data

@article{Morris2008PinnacleAF,
  title={Pinnacle: a fast, automatic and accurate method for detecting and quantifying protein spots in 2-dimensional gel electrophoresis data},
  author={Jeffrey S. Morris and Brittan N. Clark and Howard B. Gutstein},
  journal={Bioinformatics},
  year={2008},
  volume={24 4},
  pages={529-36}
}
MOTIVATION One of the key limitations for proteomic studies using 2-dimensional gel electrophoresis (2DE) is the lack of rapid, robust and reproducible methods for detecting, matching and quantifying protein spots. The most commonly used approaches involve first detecting spots and drawing spot boundaries on individual gels, then matching spots across gels and finally quantifying each spot by calculating normalized spot volumes. This approach is time consuming, error-prone and frequently… CONTINUE READING

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