Pine somatic embryogenesis using zygotic embryos as explants.

  title={Pine somatic embryogenesis using zygotic embryos as explants.},
  author={Gerald S. Pullman and Kylie Bucalo},
  journal={Methods in molecular biology},
Somatic embryogenesis (SE) has the potential to be the lowest-cost method to rapidly produce large numbers of high-value somatic seedlings with desired characteristics for plantation forestry. At least 24 of the 115-120 known Pinus species can undergo SE. Initiation for most species works best with immature megagametophytes as starting material, although a few pines can initiate SE cultures from isolated mature seed embryos. Successful initiation depends heavily on explant type, embryo… CONTINUE READING
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