Pilot sequencing of onion genomic DNA reveals fragments of transposable elements, low gene densities, and significant gene enrichment after methyl filtration

@article{Jake2008PilotSO,
  title={Pilot sequencing of onion genomic DNA reveals fragments of transposable elements, low gene densities, and significant gene enrichment after methyl filtration},
  author={Jernej Jak{\vs}e and Jenelle D. F. Meyer and Go Suzuki and John McCallum and Foo Cheung and Christopher D. Town and Michael J Havey},
  journal={Molecular Genetics and Genomics},
  year={2008},
  volume={280},
  pages={287-292}
}
Sequencing of the onion (Allium cepa) genome is challenging because it has one of the largest nuclear genomes among cultivated plants. We undertook pilot sequencing of onion genomic DNA to estimate gene densities and investigate the nature and distribution of repetitive DNAs. Complete sequences from two onion BACs were AT rich (64.8%) and revealed long tracts of degenerated retroviral elements and transposons, similar to other larger plant genomes. Random BACs were end sequenced and only 3 of… CONTINUE READING
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