Pigment epithelium-derived factor (PEDF) binds to glycosaminoglycans: analysis of the binding site.

@article{Alberdi1998PigmentEF,
  title={Pigment epithelium-derived factor (PEDF) binds to glycosaminoglycans: analysis of the binding site.},
  author={Elena M Alberdi and Christian C Hyde and S. Patricia Becerra},
  journal={Biochemistry},
  year={1998},
  volume={37 30},
  pages={10643-52}
}
Pigment epithelium-derived factor (PEDF), a neurotrophic protein, is a secreted serpin identified in extracellular matrixes. We show that PEDF extractions from the interphotoreceptor matrix are more efficient with increasing NaCl concentrations, indicating that ionic interactions mediate its association with this polyanionic matrix. We have used affinity chromatography and ultrafiltration to probe for direct binding of PEDF to glycosaminoglycans/polyanions. Correctly folded PEDF bound to… CONTINUE READING

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