Comprehensive and accurate tracking of carbon origin of LC-tandem mass spectrometry collisional fragments for 13C-MFA
- Jannick Kappelmann, Bianca Klein, Petra Geilenkirchen, Stephan Noack
- Analytical and bioanalytical chemistry
- 2017
1 Excerpt
Pig liver pyruvate carboxylase. Purification, properties and cation specificity.
- Published 1974 in The Biochemical journal
Abstract
1. Pyruvate carboxylase was purified to apparent homogeneity from pig liver mitochondria and shown to be free of all kinetically contaminating enzymes. 2. The enzyme has a mol. wt. of 520000 and is composed of four subunits, each with a mol. wt. of 130000. 3. The enzyme can exist as the active tetramer, dimer and monomer, although the tetramer appears to be… (More)