Pig heart [35S]thiophosphoryl pyruvate dehydrogenase complexes.


Thiophosphorylation of the pig heart pyruvate dehydrogenase complex with ATP [35S] (adenosine 5'[gamma-thio]-triphosphate) was analogous to phosphorylation with [gamma-32P]ATP except that thiophosphorylation of sites 2 and 3 was more rapid. With pyruvate dehydrogenase phosphatase the rate of dethiophosphorylation was 0.5% of that of dephosphorylation… (More)


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