Pig fetal pancreatic monolayers. A model of potential use in transplantation.

@article{Simpson1990PigFP,
  title={Pig fetal pancreatic monolayers. A model of potential use in transplantation.},
  author={Ann Margaret Simpson and Bernard Edward Tuch and Paul C Vincent},
  journal={Transplantation},
  year={1990},
  volume={49 6},
  pages={1133-7}
}
Endocrine-rich monolayers of pig fetal pancreas that are free of fibroblasts have been established with the ultimate aim of providing guidelines for the culture of the human equivalent. The immunogenic potential of the monolayers--hence their capacity to be grafted--has also been analyzed. Fetuses ranging from 50 to 90 days were used, and, following digestion with collagenase (4 mg/ml, 15-20 min), the pancreatic suspension was plated onto tissue culture vessels containing RPMI 1640. The fetal… CONTINUE READING