Piezo controlled microinjection: an in vivo complement for in vitro sensory studies in insects.

@article{Olsson2011PiezoCM,
  title={Piezo controlled microinjection: an in vivo complement for in vitro sensory studies in insects.},
  author={Shannon B. Olsson and Merid Negash Getahun and Dieter Wicher and Bill S. Hansson},
  journal={Journal of neuroscience methods},
  year={2011},
  volume={201 2},
  pages={385-9}
}
Recent insights into insect olfactory signaling based on in vitro analyses have created an urgent need for equivalent in vivo analyses using living organisms. Here, we present a microinjection system that establishes a "virtual petri dish" within sensory structures for the application of agents to sensory neurons. Our system uses a series of pumps to inject chemical agents via air pressure into the surrounding lymph. We show using tetrodotoxin and forskolin application that robust effects on… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 24 references

On the function of the pheromone binding protein in the olfactory hairs of Antheraea polyphemus

  • M Van den Berg, G. Ziegelberger
  • J Insect Phys
  • 1991
Highly Influential
1 Excerpt

Drosophila odorant receptors are both ligand-gated and cyclic-nucleotideexpression of the DSC1 channel

  • D Wicher, R Schäfer, +4 authors B. Hansson
  • Insect Biochem Mol Biol
  • 2011

Similar Papers

Loading similar papers…