Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells.

  title={Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells.},
  author={Marc Tramier and Isabelle Gautier and Tristan Piolot and Sylvie Ravalet and Klaus Kemnitz and Jacques Coppey and Christiane Durieux and Vincent Mignotte and Ma{\"i}t{\'e} Coppey-Moisan},
  journal={Biophysical journal},
  volume={83 6},
By using a novel time- and space-correlated single-photon counting detector, we show that fluorescence resonance energy transfer (FRET) between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) fused to herpes simplex virus thymidine kinase (TK) monomers can be used to reveal homodimerization of TK in the nucleus and cytoplasm of live cells. However, the quantification of energy transfer was limited by the intrinsic biexponential fluorescence decay of the donor CFP (lifetimes… CONTINUE READING