Picometer-scale dynamical observations of individual membrane proteins: the case of bacteriorhodopsin.

@article{Okumura2004PicometerscaleDO,
  title={Picometer-scale dynamical observations of individual membrane proteins: the case of bacteriorhodopsin.},
  author={Yasuaki Okumura and Toshihiko Oka and Mikio Kataoka and Yoshio Taniguchi and Yuji C Sasaki},
  journal={Physical review. E, Statistical, nonlinear, and soft matter physics},
  year={2004},
  volume={70 2 Pt 1},
  pages={
          021917
        }
}
In vivo measurements of dynamical conformational changes in single biomolecules under functional conditions have had a tremendous impact on molecular and cell biology. However, even single-molecule fluorescent resonance energy transfer cannot easily monitor the intramolecular dynamics in cell systems due to shortcomings in monitoring precision. Here, we report dynamical observations of irreversible intramolecular conformational changes in a single-membrane protein [bacteriorhodopsin (BR)] using… CONTINUE READING