Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement

@article{Zhang2003PichiaPF,
  title={Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement},
  author={Wenhui Zhang and Karen J. Hywood Potter and Bradley A. Plantz and Vicki L. Schlegel and L. A. Smith and Michael M. Meagher},
  journal={Journal of Industrial Microbiology and Biotechnology},
  year={2003},
  volume={30},
  pages={210-215}
}
Fed-batch fermentation of a methanol utilization plus (Mut+) Pichia pastoris strain typically has a growth phase followed by a production phase (induction phase). In the growth phase glycerol is usually used as carbon for cell growth while in the production phase methanol serves as both inducer and carbon source for recombinant protein expression. Some researchers employed a mixed glycerol-methanol feeding strategy during the induction phase to improve production, but growth kinetics on… CONTINUE READING