Phytotoxic protein PcF, purification, characterization, and cDNA sequencing of a novel hydroxyproline-containing factor secreted by the strawberry pathogen Phytophthora cactorum.

@article{Orsomando2001PhytotoxicPP,
  title={Phytotoxic protein PcF, purification, characterization, and cDNA sequencing of a novel hydroxyproline-containing factor secreted by the strawberry pathogen Phytophthora cactorum.},
  author={Giuseppe Orsomando and Maria Lorenzi and Nadia Raffaelli and M Dalla Rizza and Bruno Mezzetti and Silverio Ruggieri},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 24},
  pages={21578-84}
}
A novel protein factor, named PcF, has been isolated from the culture filtrate of Phytophthora cactorum strain P381 using a highly sensitive leaf necrosis bioassay with tomato seedlings. Isolated PcF protein alone induced leaf necrosis on its host strawberry plant. The primary structure and cDNA sequence of this novel phytotoxic protein was determined, and BLAST searches of Swiss-Prot, EMBL, and GenBank(TM)/EBI data banks showed that PcF shared no significant homology with other known sequences… CONTINUE READING
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